β-Caryophyllene

β-Caryophyllene is the only terpene in nature that directly activates cannabinoid receptors. The FDA says it's a food additive. Pharmacologically, it's a cannabinoid.

In 2008, researchers at ETH Zurich discovered that β-caryophyllene (BCP) directly binds to CB2 receptors with a binding ...

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The Cannabinoid That Slipped Through

In 2008, researchers at ETH Zurich discovered that β-caryophyllene (BCP) directly binds to CB2 receptors with a binding affinity of Ki = 155 nM. That's not 'influences' or 'modulates' — it's a full selective agonist operating through the same receptor system as THC and CBD.

This makes BCP unique among the 30,000+ terpenes in nature. No other dietary compound directly activates cannabinoid receptors. The FDA classifies it as GRAS (Generally Recognized as Safe) — you've been eating it your whole life in black pepper, cloves, oregano, hops. A cannabinoid hiding on your spice rack.

The difference: BCP targets CB2 (immune, anti-inflammatory) with zero CB1 activity (no psychoactive effects). You get cannabinoid benefits without the high. When a strain feels 'grounded' versus 'floaty,' when you feel body presence versus 'did I smoke?' — that's often the caryophyllene difference.

What the Internet Gets Wrong

What the Internet Gets Wrong
Caryophyllene's boiling point is 119-130°C (246-266°F), making it one of the first terpenes to vaporize. You need low temperatures to preserve it.
That's the melting point of impure samples. BCP's actual boiling point is 263°C (505°F). It's LESS volatile than most monoterpenes — it persists well in flower and concentrates. The optimal vaping temperature (~165°C/320°F) works because terpenes vaporize below their boiling points via vapor pressure effects.
The 'hard to vape' concern is backwards. BCP is actually quite stable. You're not losing it in the first hit.

What the Research Actually Shows

Caryophyllene has the strongest mechanistic evidence of any cannabis terpene — because it's operating through a known receptor system.

Direct CB2 receptor agonist (Ki = 155 nM)
First identification of BCP as dietary cannabinoid. Full selective agonist with functional EC50 = 1.9 μM. Zero CB1 binding.
Gertsch et al., PNAS 2008
☑☑☑☑☑A
The only terpene that does this. Binding confirmed via π-π stacking with specific residues (F117, W258) in the CB2 pocket.
Anxiolytic effects are completely CB2-mediated
CB2 antagonist (AM630) fully blocked anxiolytic effect. GABA-A antagonist (flumazenil) had no effect. 5-HT1A antagonist had no effect.
Bahi et al., 2014
☑☑☑☑☑A
Different mechanism than benzos or SSRIs. Not sedating — locomotor activity unchanged.
Crosses blood-brain barrier via inhalation
Detected in olfactory bulb and brain tissue, persisting 24+ hours. Dual pathway: pulmonary absorption + direct nasal-to-brain.
Kiyofuji et al., Sci Rep 2021
☑☑☑☑☑A
Rare for dietary compounds. Accumulates highest in brown adipose tissue — may explain 'body warmth' sensation.
Also activates PPARα and PPARγ receptors
Dual CB2 + PPAR activation explains broader effects than pure CB2 agonists.
Wu et al., Bioorg Med Chem Lett 2014
☑☑☑☑☑A
Two major anti-inflammatory pathways. More complex pharmacology than a single-target compound.
Anticonvulsant without cognitive impairment
Seizure protection while IMPROVING object recognition performance in same animals.
Multiple studies (PTZ, MES, kainic acid)
☑☑☑☑☑A
Most seizure meds impair memory. BCP doesn't. Cognitive function improved, not worsened.
Human clinical trials
Zero published efficacy trials as of 2026. Human pharmacokinetic data exists; efficacy data doesn't.
☑☑☐☐☐D
All therapeutic claims extrapolated from rodent and cell models. The translation gap is massive.

Why CB2 Matters

CB2 receptors are concentrated in immune cells, gut, and peripheral tissues — with significant but lower expression in brain microglia. Here's what BCP's CB2 agonism does.

Non-Psychoactive by Design
CB1 produces intoxication. CB2 doesn't. BCP activates CB2 with zero CB1 binding — which is why it contributes body effects without altering your mental state directly. The 'grounding' is real; the 'high' comes from THC.
Anti-Inflammatory Cascade
CB2 activation inhibits NF-κB, reduces pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and shifts microglia from M1 (inflammatory) to M2 (protective) states. This is why BCP shows up in pain, neuroprotection, and autoimmune research.
Anxiolytic Without Sedation
Most anxiolytics work through GABA (sedating) or serotonin (slow onset). BCP's anxiolysis is CB2-mediated — a completely different pathway. Reduced anxiety with unchanged motor activity in every study.
Brown Fat Accumulation
After inhalation, BCP accumulates highest in brown adipose tissue — metabolically active fat that regulates thermogenesis. The 'body warmth' of high-caryophyllene strains may have a literal metabolic basis.

High-Caryophyllene Strains

Caryophyllene is often the dominant terpene in cannabis — up to 37% of the essential oil fraction. Look for the spicy, peppery, woody notes.

Girl Scout Cookiesclassic BCP anchor
OG Kush
Bubba Kush
Chemdawg
Sour Diesel
Death Star
Skywalker OG
Original Glue (GG4)very high BCP
Purple Punch
Master Kush

BCP almost always co-occurs with humulene — they share a biosynthetic pathway. Humulene provides NF-κB inhibition; BCP provides CB2 agonism. Together: synergistic anti-inflammatory.

How Karl Tracks This

Caryophyllene is the anchor. It's the threshold that determines whether a session feels grounded or leaves you asking 'did I smoke?' Karl tracks this religiously because it's the most consistent predictor of body presence.

Below 0.5% caryophyllene: 'did I smoke?' territory. The psychoactive effects happen but there's no body registration, no proprioceptive confirmation. It feels incomplete, floaty, sometimes anxiety-inducing because you're not anchored.

Above 0.8%: solid anchor. The body knows it consumed something. Grounded, stable, present. Above 1.5%: brain hug territory — that embracing body warmth that defines the best evening sessions.

anchor:Sufficient caryophyllene to ground heady/spacey effects. Body registration that confirms consumption. Minimum threshold: 0.5%.
brain hug:Strong caryophyllene presence (≥1.5%) — full body awareness, warmth, embracing presence without sedation or fog.
0.5% minimum / 0.8% solid / 1.5% brain hug
Three-tier anchor system. Below 0.5% risks ungrounded dissociation. 0.8%+ provides reliable presence. 1.5%+ delivers the full body embrace.

What This Means For You

If strains feel 'incomplete' or 'heady only'
Check caryophyllene content. 'Did I smoke?' experiences often correlate with BCP below 0.5%. Look for 0.8%+ for reliable grounding.
For anxiety without sedation
High-caryophyllene strains provide CB2-mediated calm that doesn't impair function. Different mechanism than GABAergic terpenes — calm without drowsiness.
For pain and inflammation
BCP's dual CB2 + PPAR activation makes it the most mechanistically supported terpene for inflammatory conditions. Look for 1.0%+ and pair with humulene for synergy.
With high limonene
Watch for 'ungrounded limonene' — limonene >2% with caryophyllene <0.5% can feel jittery or anxious. The anchor balances the lift.

Related Terpenes

Continue exploring the science behind terpenes.

Caryophyllene is the anchor that makes or breaks a strain. Your body knows when it's missing. Karl tracks what grounds you.